HoxD12 (homeobox D12), also known as HOX4H, is a 275aa protein that localizes to the nucleus and contains one homeobox DNA-binding domain. One of several members of the homeobox superfamily, HoxD12 functions as a sequence specific transcription factor that is important for the correct positioning of developing limb buds on the anterior-posterior axis. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 813bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTGTGAGCGCAGTCTCTACAGAGCGGGCTATGTGGGCTCGCTTCTGAATCTGCAGTCGCCAGACTCTTTCTACTTCTCCAACCTGAGGCCGAATGGCGGCCAGTTGGCCGCGCTTCCCCCTATCTCCTACCCGCGCGGCGCGCTGCCCTGGGCCGCCACGCCCGCCTCCTGCGCCCCCGCGCAGCCTGCGGGCGCCACTGCCTTCGGCGGCTTCTCGCAGCCCTACCTGGCTGGCTCCGGGCCTCTCGGCCTGCAGCCCCCAACAGCCAAAGACGGACCCGAAGAGCAGGCTAAGTTCTATGCGCCCGAAGCGGCCGCTGGGCCAGAGGAGCGCGGTCGTACCCGGCCGTCCTTCGCCCCCGAGTCTAGCCTGGCTCCTGCAGTGGCTGCTCTCAAAGCGGCCAAGTATGACTACGCTGGTGTGGGTCGTGCCACGCCGGGCTCCACGACCCTGCTCCAGGGGGCTCCCTGCGCCCCTGGCTTCAAGGACGACACCAAGGGCCCGCTCAACTTGAACATGACAGTGCAGGCGGCGGGCGTTGCCTCTTGCCTGCGACCTTCACTGCCCGACGGCCTGCCGTGGGGGGCGGCCCCGGGGAGGGCCCGCAAGAAGCGGAAACCCTACACGAAGCAGCAGATTGCGGAGTTGGAGAACGAATTCCTCGTCAACGAATTCATCAACAGGCAGAAACGCAAGGAATTGTCCAATAGGCTGAACCTCAGCGACCAGCAAGTCAAAATCTGGTTCCAGAACAGGCGTATGAAGAAGAAGCGCGTGGTGCTTCGGGAGCAGGCGCTGGCGCTCTACTAG Translation Sequence: MCERSLYRAG YVGSLLNLQS PDSFYFSNLR PNGGQLAALP PISYPRGALP WAATPASCAP AQPAGATAFG GFSQPYLAGS GPLGLQPPTA KDGPEEQAKF YAPEAAAGPE ERGRTRPSFA PESSLAPAVA ALKAAKYDYA GVGRATPGST TLLQGAPCAP GFKDDTKGPL NLNMTVQAAG VASCLRPSLP DGLPWGAAPG RARKKRKPYT KQQIAELENE FLVNEFINRQ KRKELSNRLN LSDQQVKIWF QNRRMKKKRV VLREQALALY Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
