HSF2 belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1611bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAAGCAGAGTTCGAACGTGCCGGCTTTCCTCAGCAAGCTGTGGACGCTTGTGGAGGAAACCCACACTAACGAGTTCATCACCTGGAGCCAGAATGGCCAAAGTTTTCTGGTCTTGGATGAGCAACGATTTGCAAAAGAAATTCTTCCCAAATATTTCAAGCACAATAATATGGCAAGCTTTGTGAGGCAACTGAATATGTATGGTTTCCGTAAAGTAGTACATATCGACTCTGGAATTGTAAAGCAAGAAAGAGATGGTCCTGTAGAATTTCAGCATCCTTACTTCAAACAAGGACAGGATGACTTGTTGGAGAACATTAAAAGGAAGGTTTCATCTTCAAAACCAGAAGAAAATAAAATTCGTCAGGAAGATTTAACAAAAATTATAAGTAGTGCTCAGAAGGTTCAGATAAAACAGGAAACTATTGAGTCCAGGCTTTCTGAATTAAAAAGTGAGAATGAGTCCCTTTGGAAGGAGGTGTCAGAATTACGAGCAAAGCATGCACAACAGCAACAAGTTATTCGAAAGATTGTCCAGTTTATTGTTACATTGGTTCAAAATAACCAACTTGTGAGTTTAAAACGTAAAAGGCCTCTACTTCTAAACACTAATGGAGCCCAAAAGAAGAACCTGTTTCAGCACATAGTCAAAGAACCAACTGATAATCATCATCATAAAGTTCCACACAGTAGGACTGAAGGTTTAAAGCCAAGGGAGAGGATTTCAGATGACATCATTATTTATGATGTTACTGATGATAATGCAGATGAAGAAAATATCCCAGTTATTCCAGAAACTAATGAGGATGTTATATCTGATCCCTCCAACTGTAGCCAGTACCCTGATATTGTCATCGTTGAAGATGACAATGAAGATGAGTATGCACCTGTCATTCAGAGTGGAGAGCAGAATGAACCAGCCAGAGAATCCCTAAGTTCAGGCAGTGATGGCAGCAGCCCTCTCATGTCTAGTGCTGTCCAGCTAAATGGCTCATCCAGTCTGACCTCAGAAGATCCAGTGACCATGATGGATTCCATTTTGAATGATAACATCAATCTTTTGGGAAAGGTTGAGCTGTTGGATTATCTTGACAGTATTGACTGCAGTTTAGAGGACTTCCAGGCCATGCTATCAGGAAGACAATTTAGCATAGACCCAGATCTCCTGGTTGATCTTTTCACTAGTTCTGTGCAGATGAATCCCACAGATTACATCAATAATACAAAATCTGAGAATAAAGGATTAGAAACTACCAAGAACAATGTAGTTCAGCCAGTTTCGGAAGAGGGAAGAAAATCTAAATCCAAACCAGATAAGCAGCTTATCCAGTATACCGCCTTTCCACTTCTTGCATTCCTCGATGGGAACCCTGCTTCTTCTGTTGAACAGGCGAGTACAACAGCATCATCAGAAGTTTTGTCCTCTGTAGATAAACCCATAGAAGTTGATGAGCTTCTGGATAGCAGCCTAGACCCAGAACCAACCCAAAGTAAGCTTGTTCGCCTGGAGCCATTGACTGAAGCTGAAGCTAGTGAAGCTACACTGTTTTATTTATGTGAACTTGCTCCTGCACCTCTGGATAGTGATATGCCACTTTTAGATAGCTAA Translation Sequence: MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP KYFKHNNMAS FVRQLNMYGF RKVVHIDSGI VKQERDGPVE FQHPYFKQGQ DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ ETIESRLSEL KSENESLWKE VSELRAKHAQ QQQVIRKIVQ FIVTLVQNNQ LVSLKRKRPL LLNTNGAQKK NLFQHIVKEP TDNHHHKVPH SRTEGLKPRE RISDDIIIYD VTDDNADEEN IPVIPETNED VISDPSNCSQ YPDIVIVEDD NEDEYAPVIQ SGEQNEPARE SLSSGSDGSS PLMSSAVQLN GSSSLTSEDP VTMMDSILND NINLLGKVEL LDYLDSIDCS LEDFQAMLSG RQFSIDPDLL VDLFTSSVQM NPTDYINNTK SENKGLETTK NNVVQPVSEE GRKSKSKPDK QLIQYTAFPL LAFLDGNPAS SVEQASTTAS SEVLSSVDKP IEVDELLDSS LDPEPTQSKL VRLEPLTEAE ASEATLFYLC ELAPAPLDSD MPLLDS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
