HSPB9, also known as CT51, belongs to the small heat shock protein (HSP20) family. Localizing to the cytoplasm as well as the nucleus, HSPB9 is expressed specifically in the testis. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 480bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCAGAGAG TCGGTAACAC CTTCTCCAAC GAGAGCCGGG TGGCATCCCG GTGTCCCAGC GTGGGCCTTG CTGAACGGAA CCGGGTGGCC ACAATGCCGG TGCGGCTGCT CAGGGACAGT CCAGCGGCTC AGGAGGACAA TGACCATGCC AGAGACGGTT TCCAAATGAA GCTGGATGCC CACGGCTTCG CCCCGGAGGA ACTGGTGGTG CAGGTGGATG GCCAATGGCT GATGGTGACC GGACAGCAGC AACTGGACGT CAGGGACCCG GAAAGGGTCA GTTACCGCAT GTCACAGAAG GTGCACCGGA AAATGCTCCC GTCCAACCTG AGTCCTACCG CCATGACCTG CTGCCTGACC CCCTCCGGGC AGCTGTGGGT CAGAGGCCAG TGTGTGGCGC TGGCCCTCCC TGAAGCCCAA ACAGGACCGT CCCCGAGACT CGGGAGCCTC GGCTCTAAGG CTTCCAACCT GACCCGGTAA Translation Sequence: MQRVGNTFSN ESRVASRCPS VGLAERNRVA TMPVRLLRDS PAAQEDNDHA RDGFQMKLDA HGFAPEELVV QVDGQWLMVT GQQQLDVRDP ERVSYRMSQK VHRKMLPSNL SPTAMTCCLT PSGQLWVRGQ CVALALPEAQ TGPSPRLGSL GSKASNLTR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
