HuS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. This protein associates with Rad9 and Rad1 to form the 9-1-1 (RAD9-RAD1-HuS1) complex, which localizes to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to EcoR I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 843bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAAGTTTC GGGCCAAGAT CGTGGACGGG GCCTGTCTGA ACCACTTCAC ACGAATCAGT AACATGATAG CCAAGCTTGC CAAAACCTGC ACCCTCCGCA TCAGCCCTGA TAAGCTTAAC TTCATCCTTT GTGACAAGCT GGCTAATGGA GGAGTGAGCA TGTGGTGTGA GCTGGAACAG GAGAACTTCT TCAACGAATT TCAAATGGAG GGTGTCTCTG CAGAAAACAA TGAGATTTAT TTAGAGCTAA CATCGGAAAA CTTATCTCGA GCCTTGAAGA CTGCCCAGAA TGCCAGGGCT TTGAAAATCA AACTGACTAA TAAACACTTT CCCTGCCTCA CGGTCTCCGT GGAGCTGTTA TCTATGTCAA GCAGTAGCCG CATTGTGACC CATGACATCC CCATAAAGGT GATTCCTAGG AAATTGTGGA AGGACTTACA AGAACCGGTG GTCCCAGATC CTGATGTTAG TATTTATTTA CCAGTCTTGA AGACTATGAA GAGTGTTGTG GAAAAAATGA AAAACATCAG CAATCACCTT GTTATTGAAG CAAACCTAGA TGGAGAATTG AATTTGAAAA TAGAAACTGA ATTAGTATGT GTTACAACTC ATTTTAAAGA TCTTGGAAAT CCTCCATTAG CCTCTGAAAG CACCCATGAG GACAGAAACG TGGAACACAT GGCTGAAGTG CACATAGATA TTAGGAAGCT CCTACAGTTT CTTGCTGGAC AACAAGTAAA TCCCACAAAG GCCTTATGCA ATATTGTGAA TAACAAGATG GTGCATTTTG ATCTGCTTCA TGAAGACGTG TCCCTTCAGT ATTTCATCCC TGCGCTGTCC TAG Translation Sequence: MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
