HYLS1 encodes a protein localized to the cytoplasm. Mutations in this gene are associated with hydrolethalus syndrome. Multiple alternatively spliced variants, encoding the same protein, have been identified. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 900bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGAAGAACTTCTACCTGATGGACAAATATGGGCTAATATGGATCCAGAAGAACGAATGTTGGCAGCTGCTACAGCTTTTACCCACATCTGTGCAGGGCAGGGTGAAGGAGATGTCAGGAGAGAAGCCCAATCTATCCAATATGATCCCTACAGTAAAGCTTCAGTAGCCCCAGGGAAGCGACCTGCTCTTCCTGTGCAACTACAGTACCCACATGTAGAAAGTAATGTCCCTTCAGAAACAGTCTCTGAGGCCTCCCAAAGACTCCGAAAGCCAGTGATGAAGAGAAAGGTGCTGCGCAGAAAGCCAGATGGGGAAGTATTAGTAACAGATGAGTCGATTATCAGTGAATCAGAATCTGGTACAGAAAATGATCAGGATCTCTGGGACTTAAGACAAAGGCTGATGAATGTACAGTTCCAGGAAGACAAGGAATCTTCATTTGATGTTTCACAAAAATTTAACCTACCACATGAATACCAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACAAAGAGAAGGAATGGGCTCTCCAGCTTACGAACAAGACCTGATTGTTGCCAGCAGACCCAAGTCCTTTATTCTCCCAAAGCTGGACCAGTTAAGCCGAAACCGGGGCAAGACAGACCGGGTAGCCCGGTATTTTGAGTACAAACGGGACTGGGACTCAATACGTTTACCTGGTGAAGATCATAGAAAGGAATTACGCTGGGGTGTCCGAGAGCAGATGCTTTGTCGAGCAGAACCCCAATCCAAACCTCAGCATATATATGTCCCAAACAATTATCTAGTACCAACAGAGAAGAAAAGGTCTGCACTCCGTTGGGGTGTTCGTTGTGACCTTGCAAATGGTGTCATACCCAGGAAGCTTCCCTTCCCTCTTTCTCCTTCTTAA Translation Sequence: MEELLPDGQI WANMDPEERM LAAATAFTHI CAGQGEGDVR REAQSIQYDP YSKASVAPGK RPALPVQLQY PHVESNVPSE TVSEASQRLR KPVMKRKVLR RKPDGEVLVT DESIISESES GTENDQDLWD LRQRLMNVQF QEDKESSFDV SQKFNLPHEY QGISQDQLIC SLQREGMGSP AYEQDLIVAS RPKSFILPKL DQLSRNRGKT DRVARYFEYK RDWDSIRLPG EDHRKELRWG VREQMLCRAE PQSKPQHIYV PNNYLVPTEK KRSALRWGVR CDLANGVIPR KLPFPLSPS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
