IL-1 alpha /IL-1F1, Recombinant, Mouse, aa115-270, His-Tag

Product Overview

• Article Name: IL-1 alpha /IL-1F1, Recombinant, Mouse, aa115-270, His-Tag
• Biozol Catalog Number: USB-552027
• Supplier Catalog Number: 552027
• Alternative Catalog Number: USB-552027-50,USB-552027-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

IL-1 alpha /IL-1F1, also known as interleukin-1 alpha, is a member of the interleukin-1 cytokine family. Cytokines are protein signaling compounds that are the main mediators of the immune response. This protein is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. Also, it stimulates thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation. Recombinant mouse IL-1 alpha, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa115-270 from mouse IL-1 alpha /IL-1F1 protein, fused His-Tag at C-terminal, was expressed in baculovirus. AA Sequence: SAPYTYQ SDLRYKLMKL VRQKFVMNDS LNQTIYQDVD KHYLSTTWLN DLQQEVKFDM YAYSSGGDDS KYPVTLKISD SQLFVSAQGE DQPVLLKELP ETPKLITGSE TDLIFFWKSI NSKNYFTSAA YPELFIATKE QSRVHLARGL PSMTDFQIS Biological Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range 0.15ng/ml. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 19
• NCBI: 034684
• UniProt: P01582
• Purity: 90% (SDS-PAGE). Endotoxin: <1EU/1ug (LAL)
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.