IMP4 forms a ternary complex with IMP3 and MPP10 that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 876bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCTGCGCCGCGAGGCCCGCCTGCGCCGCGAGTACCTGTACCGCAAGGCCCGGGAGGAGGCGCAGCGCTCAGCCCAGGAGAGGAAGGAGCGGCTGCGGCGCGCGCTGGAAGAAAACCGCCTGATTCCCACTGAGTTACGCCGAGAGGCTCTGGCCTTACAGGGGTCCCTGGAGTTTGATGATGCTGGAGGTGAAGGTGTGACCAGCCACGTGGATGATGAATACCGATGGGCAGGAGTCGAGGATCCCAAGGTTATGATCACTACCTCCCGAGACCCCAGTTCCCGCCTCAAGATGTTTGCAAAGGAGCTGAAGCTGGTGTTCCCGGGCGCCCAGCGAATGAACCGAGGTCGACATGAAGTGGGGGCACTGGTGCGAGCCTGCAAAGCCAACGGCGTCACCGATCTGCTGGTCGTTCACGAGCATCGGGGCACACCTGTGGGGCTCATCGTCAGCCACCTGCCCTTTGGTCCTACTGCCTACTTCACGCTGTGCAATGTGGTCATGCGGCATGACATCCCAGACCTGGGCACCATGTCGGAGGCCAAGCCCCACCTCATCACACACGGCTTCTCCTCCCGCCTGGGCAAGCGGGTCTCTGACATCCTCCGATACCTATTTCCCGTGCCCAAAGATGACAGCCACCGGGTCATCACCTTCGCAAACCAGGACGACTACATATCATTCCGGCACCATGTGTATAAGAAGACAGACCACCGCAACGTGGAGCTCACTGAGGTCGGGCCCCGCTTTGAGCTGAAGCTGTACATGATCCGTCTGGGCACGCTGGAGCAGGAGGCCACAGCAGACGTGGAGTGGCGCTGGCACCCTTACACCAATACCGCACGCAAGAGAGTCTTCCTGAGCACCGAGTGA Translation Sequence: MLRREARLRR EYLYRKAREE AQRSAQERKE RLRRALEENR LIPTELRREA LALQGSLEFDDAGGEGVTSH VDDEYRWAGV EDPKVMITTS RDPSSRLKMF AKELKLVFPG AQRMNRGRHEVGALVRACKA NGVTDLLVVH EHRGTPVGLI VSHLPFGPTA YFTLCNVVMR HDIPDLGTMSEAKPHLITHG FSSRLGKRVS DILRYLFPVP KDDSHRVITF ANQDDYISFR HHVYKKTDHRNVELTEVGPR FELKLYMIRL GTLEQEATAD VEWRWHPYTN TARKRVFLST E Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
