Interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. Exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV) and yellow fever virus (YFV) in an exonuclease-dependent manner. May also play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 546bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCTGGGA GCCGTGAGGT GGTGGCCATG GACTGCGAGA TGGTGGGGCT GGGGCCCCAC CGGGAGAGTG GCCTGGCTCG TTGCAGCCTC GTGAACGTCC ACGGTGCTGT GCTGTACGAC AAGTTCATCC GGCCTGAGGG AGAGATCACC GATTACAGAA CCCGGGTCAG CGGGGTCACC CCTCAGCACA TGGTGGGGGC CACACCATTT GCCGTGGCCA GGCTAGAGAT CCTGCAGCTC CTGAAAGGCA AGCTGGTGGT GGGTCATGAC CTGAAGCACG ACTTCCAGGC ACTGAAAGAG GACATGAGCG GCTACACAAT CTACGACACG TCCACTGACA GGCTGTTGTG GCGTGAGGCC AAGCTGGACC ACTGCAGGCG TGTCTCCCTG CGGGTGCTGA GTGAGCGCCT CCTGCACAAG AGCATCCAGA ACAGCCTGCT TGGACACAGC TCGGTGGAAG ATGCGAGGGC AACGATGGAG CTCTATCAAA TCTCCCAGAG AATCCGAGCC CGCCGAGGGC TGCCCCGCCT GGCTGTGTCA GACTGA Translation Sequence: MAGSREVVAM DCEMVGLGPH RESGLARCSL VNVHGAVLYD KFIRPEGEIT DYRTRVSGVT PQHMVGATPF AVARLEILQL LKGKLVVGHD LKHDFQALKE DMSGYTIYDT STDRLLWREA KLDHCRRVSL RVLSERLLHK SIQNSLLGHS SVEDARATME LYQISQRIRA RRGLPRLAVS D Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
