The protein encoded by JSRP1 is involved in excitation-contraction coupling at the sarcoplasmic reticulum. The encoded protein can interact with CACNA1S, CACNB1, and calsequestrin to help regulate calcium influx and efflux in skeletal muscle. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 996bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTCCATGACAACCAGAGCCTGGGAGGAGCTGGATGGCGGCCTGGGCAGCTGCCAGGCCCTGGAGGACCACTCTGCGCTGGCCGAGACCCAGGAGGACAGGGCTTCAGCGACACCCAGGCTGGCCGACTCCGGCAGCGTGCCCCACGACTCTCAGGTGGCTGAAGGCCCCAGTGTGGACACCAGGCCCAAGAAGATGGAAAAAGAGCCTGCCGCCAGGGGGACCCCAGGAACGGGGAAGGAGAGGCTGAAAGCCGGAGCGAGCCCTCGGAGCGTCCCCGCGCGCAAGAAGGCGCAGACCGCGCCGCCCCTGCAGCCGCCGCCGCCGCCCCCGGCCCTGAGCGAGGAGCTGCCCTGGGGAGACCTGTCGCTCAACAAGTGCCTGGTGCTCGCCTCGCTGGTGGCGCTGCTGGGCTCGGCTTTCCAGCTGTGCCGCGACGCCGTCCCTGGGGAGGCAGCACTCCAAGCACGTGTGCCCGAGCCCTGGGTCCCGCCAAGCTCAGCCCCGAGGGAGCCATCGTCGCCCCTGCCTAAGTTCGAGGCCCAGGCGCCTCCATCAGCGCCGCCTGCGCCCCGGGCCGAGGCAGAGGTCAGACCCAAGATTCCCGGGAGTCGGGAGGCTGCAGAGAACGACGAAGAGGAGCCCGGCGAGGCCACCGGAGAGGCCGTCCGGGAGGACCGTGTGACCCTCGCAGACCGGGGACCCAAGGAGAGGCCTCGGAGAGAGGGGAAGCCGCGGAAGGAGAAGCCGCGGAAGGAGGAGAGACCTAAGAAAGAGAGGCCGCGGAAAGAGGAGAGGCCACGGGCCGCCAGGGAGCCCCGGGAAGCCCTACCCCAGCGCTGGGAGTCACGCGAAGGGGGCCACCGGCCGTGGGCACGGGACTCCAGGGACGCCGAGCCCAGGAAGAAGCAGGCCTGGGTGTCCCCGAGGCGTCCCGACGAGGAGCAGCGGCCTGGGAGTCGCCAGAAGCTCCGCGCAGGCAAGGGGCGGGACTGA Translation Sequence: MSMTTRAWEE LDGGLGSCQA LEDHSALAET QEDRASATPR LADSGSVPHD SQVAEGPSVD TRPKKMEKEP AARGTPGTGK ERLKAGASPR SVPARKKAQT APPLQPPPPP PALSEELPWG DLSLNKCLVL ASLVALLGSA FQLCRDAVPG EAALQARVPE PWVPPSSAPR EPSSPLPKFE AQAPPSAPPA PRAEAEVRPK IPGSREAAEN DEEEPGEATG EAVREDRVTL ADRGPKERPR REGKPRKEKP RKEERPKKER PRKEERPRAA REPREALPQR WESREGGHRP WARDSRDAEP RKKQAWVSPR RPDEEQRPGS RQKLRAGKGR D Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
