The product encoded by LPXN is preferentially expressed in hematopoietic cells and belongs to the paxillin protein family. Similar to other members of this focal-adhesion-associated adaptor-protein family, it has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by, tyrosine kinase activity. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1176bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTCTACATTATTGATCTCGTCTTCCGATGCCTTATTGGAGGAACTGGAACGCTCCACCCTTCAGGACAGTGATGAATATTCCAACCCAGCTCCTCTTCCCCTGGATCAGCATTCCAGAAAGGAGACTAACCTTGATGAGACTTCGGAGATCCTTTCTATTCAGGATAACACAAGTCCCTTGCCGGCGCAGCTCGTGTATACTACCAATATCCAGGAGCTCAATGTCTACAGTGAAGCCCAAGAGCCAAAGGAATCACCACCACCTTCTAAAACGTCAGCAGCTGCTCAGTTGGATGAGCTCATGGCTCACCTGACTGAGATGCAGGCCAAGGTTGCAGTGAGAGCAGATGCTGGCAAGAAGCACTTACCAGACAAGCAGGATCACAAGGCCTCCCTGGACTCAATGCTTGGGGGTCTGGAGCAGGAATTGCAGGACCTTGGCATTGCCACAGTGCCCAAGGGCCATTGTGCATCCTGCCAGAAACCGATTGCTGGGAAGGTGATCCATGCTCTAGGGCAATCATGGCATCCTGAGCATTTTGTCTGTACTCATTGCAAAGAAGAGATTGGCTCCAGTCCCTTCTTTGAGCGGAGTGGCTTGGCCTACTGCCCCAACGACTACCACCAACTTTTTTCTCCACGCTGTGCTTACTGCGCTGCTCCCATCCTGGATAAAGTGCTGACAGCAATGAACCAGACCTGGCACCCAGAGCACTTCTTCTGCTCTCACTGCGGAGAGGTGTTTGGTGCAGAAGGCTTTCATGAGAAGGACAAGAAGCCATATTGCCGAAAGGATTTCTTAGCCATGTTCTCACCCAAGTGTGGTGGCTGCAATCGCCCAGTGTTGGAAAACTACCTTTCAGCCATGGACACTGTCTGGCACCCAGAGTGCTTTGTTTGTGGGGACTGCTTCACCAGTTTTTCTACTGGCTCCTTCTTTGAACTGGATGGACGTCCATTCTGTGAGCTCCATTACCATCACCGCCGGGGAACGCTCTGCCATGGGTGTGGGCAGCCCATCACTGGCCGTTGTATCAGTGCCATGGGGTACAAGTTCCATCCTGAGCACTTTGTGTGTGCTTTCTGCCTGACACAGTTGTCGAAGGGCATTTTCAGGGAGCAGAATGACAAGACCTATTGTCAACCTTGCTTCAATAAGCTCTTCCCACTGTAA Translation Sequence: MSTLLISSSD ALLEELERST LQDSDEYSNP APLPLDQHSR KETNLDETSE ILSIQDNTSP LPAQLVYTTN IQELNVYSEA QEPKESPPPS KTSAAAQLDE LMAHLTEMQA KVAVRADAGK KHLPDKQDHK ASLDSMLGGL EQELQDLGIA TVPKGHCASC QKPIAGKVIH ALGQSWHPEH FVCTHCKEEI GSSPFFERSG LAYCPNDYHQ LFSPRCAYCA APILDKVLTA MNQTWHPEHF FCSHCGEVFG AEGFHEKDKK PYCRKDFLAM FSPKCGGCNR PVLENYLSAM DTVWHPECFV CGDCFTSFST GSFFELDGRP FCELHYHHRR GTLCHGCGQP ITGRCISAMG YKFHPEHFVC AFCLTQLSKG IFREQNDKTY CQPCFNKLFP L Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
