MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1182bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGACGG CTCTGCAGTT AACGGGACCA GCTCTGCGGA GACCAACTTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTA GAGCTTGATG AGCAGCAGCG AAAGCGCCTT GAGGCCTTTC TTACCCAGAA GCAGAAGGTG GGAGAACTGA AGGATGACGA CTTTGAGAAG ATCAGTGAGC TGGGGGCTGG CAATGGCGGT GTGGTGTTCA AGGTCTCCCA CAAGCCTTCT GGCCTGGTCA TGGCCAGAAA GCTAATTCAT CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATAAGGG AGCTGCAGGT TCTGCATGAG TGCAACTCTC CGTACATCGT GGGCTTCTAT GGTGCGTTCT ACAGCGATGG CGAGATCAGT ATCTGCATGG AGCACATGGA TGGAGGTTCT CTGGATCAAG TCCTGAAGAA AGCTGGAAGA ATTCCTGAAC AAATTTTAGG AAAAGTTAGC ATTGCTGTAA TAAAAGGCCT GACATATCTG AGGGAGAAGC ACAAGATCAT GCACAGAGAT GTCAAGCCCT CCAACATCCT AGTCAACTCC CGTGGGGAGA TCAAGCTCTG TGACTTTGGG GTCAGCGGGC AGCTCATCGA CTCCATGGCC AACTCCTTCG TGGGCACAAG GTCCTACATG TCGCCAGAAA GACTCCAGGG GACTCATTAC TCTGTGCAGT CAGACATCTG GAGCATGGGA CTGTCTCTGG TAGAGATGGC GGTTGGGAGG TATCCCATCC CTCCTCCAGA TGCCAAGGAG CTGGAGCTGA TGTTTGGGTG CCAGGTGGAA GGAGATGCGG CTGAGACCCC ACCCAGGCCA AGGACCCCCG GGAGGCCCCT TAGCTCATAC GGAATGGACA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT AGTCAACGAG CCTCCTCCAA AACTGCCCAG TGGAGTGTTC AGTCTGGAAT TTCAAGATTT TGTGAATAAA TGCTTAATAA AAAACCCCGC AGAGAGAGCA GATTTGAAGC AACTCATGGT TCATGCTTTT ATCAAGAGAT CTGATGCTGA GGAAGTGGAT TTTGCAGGTT GGCTCTGCTC CACCATCGGC CTTAACCAGC CCAGCACACC AACCCATGCT GCTGGCGTCT AA Translation Sequence: MPKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA AGV Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
