MAP2K2, also known as MEK2, belongs to the STE grouping of kinases. Both MEK2 and the related MEK1 are dualspecificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. MAP2K2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1203bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCTGGCCC GGAGGAAGCC GGTGCTGCCG GCGCTCACCA TCAACCCTAC CATCGCCGAG GGCCCATCCC CTACCAGCGA GGGCGCCTCC GAGGCAAACC TGGTGGACCT GCAGAAGAAG CTGGAGGAGC TGGAACTTGA CGAGCAGCAG AAGAAGCGGC TGGAAGCCTT TCTCACCCAG AAAGCCAAGG TCGGCGAACT CAAAGACGAT GACTTCGAAA GGATCTCAGA GCTGGGCGCG GGCAACGGCG GGGTGGTCAC CAAAGTCCAG CACAGACCCT CGGGCCTCAT CATGGCCAGG AAGCTGATCC ACCTTGAGAT CAAGCCGGCC ATCCGGAACC AGATCATCCG CGAGCTGCAG GTCCTGCACG AATGCAACTC GCCGTACATC GTGGGCTTCT ACGGGGCCTT CTACAGTGAC GGGGAGATCA GCATTTGCAT GGAACACATG GACGGCGGCT CCCTGGACCA GGTGCTGAAA GAGGCCAAGA GGATTCCCGA GGAGATCCTG GGGAAAGTCA GCATCGCGGT TCTCCGGGGC TTGGCGTACC TCCGAGAGAA GCACCAGATC ATGCACCGAG ATGTGAAGCC CTCCAACATC CTCGTGAACT CTAGAGGGGA GATCAAGCTG TGTGACTTCG GGGTGAGCGG CCAGCTCATC GACTCCATGG CCAACTCCTT CGTGGGCACG CGCTCCTACA TGGCTCCGGA GCGGTTGCAG GGCACACATT ACTCGGTGCA GTCGGACATC TGGAGCATGG GCCTGTCCCT GGTGGAGCTG GCCGTCGGAA GGTACCCCAT CCCCCCGCCC GACGCCAAAG AGCTGGAGGC CATCTTTGGC CGGCCCGTGG TCGACGGGGA AGAAGGAGAG CCTCACAGCA TCTCGCCTCG GCCGAGGCCC CCCGGGCGCC CCGTCAGCGG TCACGGGATG GATAGCCGGC CTGCCATGGC CATCTTTGAA CTCCTGGACT ATATTGTGAA CGAGCCACCT CCTAAGCTGC CCAACGGTGT GTTCACCCCC GACTTCCAGG AGTTTGTCAA TAAATGCCTC ATCAAGAACC CAGCGGAGCG GGCGGACCTG AAGATGCTCA CAAACCACAC CTTCATCAAG CGGTCCGAGG TGGAAGAAGT GGATTTTGCC GGCTGGTTGT GTAAAACCCT GCGGCTGAAC CAGCCCGGCA CACCCACGCG CACCGCCGTG TGA Translation Sequence: MLARRKPVLP ALTINPTIAE GPSPTSEGAS EANLVDLQKK LEELELDEQQ KKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKVQ HRPSGLIMAR KLIHLEIKPA IRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEEIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMAPERLQ GTHYSVQSDI WSMGLSLVEL AVGRYPIPPP DAKELEAIFG RPVVDGEEGE PHSISPRPRP PGRPVSGHGM DSRPAMAIFE LLDYIVNEPP PKLPNGVFTP DFQEFVNKCL IKNPAERADL KMLTNHTFIK RSEVEEVDFA GWLCKTLRLN QPGTPTRTAV Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
