This gene is a transcriptional repressor capable of binding an E-box element either as a homodimer or as a heterodimer with E2A in vitro. The encoded protein also forms heterodimers with E2A proteins in vivo. This protein is capable of inhibiting the transactivation capability of E47, an E2A protein, in mammalian cells. This gene is a downstream target of the B-cell receptor signal transduction pathway. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 621bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTCCACGG GCTCGGTGAG TGATCCGGAG GAGATGGAGC TTCGGGGGCT GCAGCGGGAG TACCCGGTCC CCGCCTCCAA GAGGCCGCCC CTCCGCGGCG TAGAGCGCAG CTACGCCTCG CCCAGTGACA ACTCGTCGGC AGAGGAGGAG GACCCCGACG GCGAGGAGGA GCGCTGCGCT CTGGGCACAG CCGGCAGCGC GGAAGGCTGC AAGAGGAAGC GGCCCCGTGT GGCTGGGGGC GGCGGCGCAG GTGGTAGCGC GGGCGGTGGT GGCAAGAAGC CCCTCCCGGC CAAGGGCTCA GCCGCAGAGT GCAAGCAGTC GCAGCGGAAC GCGGCCAACG CCCGTGAGCG TGCCCGGATG CGCGTGCTGA GCAAAGCCTT CTCCAGGCTC AAGACCAGCC TGCCCTGGGT GCCCCCCGAC ACTAAGCTCT CCAAGCTGGA CACGCTCCGG CTGGCTTCCA GTTACATCGC TCACCTGCGG CAGCTGTTGC AGGAGGACCG CTATGAGAAC GGCTACGTGC ACCCAGTGAA CCTGACATGG CCATTCGTGG TCTCGGGAAG ACCGGACTCT GACACCAAAG AAGTTTCCGC AGCCAACAGA CTATGTGGAA CCACCGCTTA A Translation Sequence: MSTGSVSDPE EMELRGLQRE YPVPASKRPP LRGVERSYAS PSDNSSAEEE DPDGEEERCA LGTAGSAEGC KRKRPRVAGG GGAGGSAGGG GKKPLPAKGS AAECKQSQRN AANARERARM RVLSKAFSRL KTSLPWVPPD TKLSKLDTLR LASSYIAHLR QLLQEDRYEN GYVHPVNLTW PFVVSGRPDS DTKEVSAANR LCGTTA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
