NDEL1 is a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 987bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGATGGTGAAGATATACCAGATTTTTCAAGTTTAAAGGAGGAAACTGCTTATTGGAAGGAACTTTCCTTGAAGTATAAGCAAAGCTTCCAGGAAGCTCGGGATGAGCTAGTTGAATTCCAGGAAGGAAGCAGAGAATTAGAAGCAGAGTTGGAGGCACAATTAGTACAGGCTGAACAAAGAAATAGAGACTTGCAGGCTGATAACCAAAGACTGAAATATGAAGTGGAGGCATTAAAGGAGAAGCTAGAGCATCAATATGCACAGAGCTATAAGCAGGTCTCAGTGTTAGAAGATGATTTAAGTCAGACTCGGGCCATTAAGGAGCAGTTGCATAAGTATGTGAGAGAGCTGGAGCAGGCCAACGACGACCTGGAGCGAGCCAAAAGGGCAACAATAGTTTCACTGGAAGACTTTGAACAAAGGCTAAACCAGGCCATTGAACGAAATGCATTTTTAGAAAGTGAACTTGATGAAAAGGAATCTTTGTTGGTCTCTGTACAGAGGTTAAAGGATGAAGCAAGAGATTTAAGGCAAGAACTAGCAGTTCGGGAAAGACAACAGGAAGTAACTAGAAAGTCGGCTCCTAGCTCTCCAACTCTAGACTGTGAAAAGATGGACTCCGCCGTCCAAGCATCACTTTCTTTGCCAGCTACCCCTGTTGGCAAAGGAACGGAGAACACTTTTCCTTCACCGAAAGCTATACCAAATGGTTTTGGTACCAGTCCACTAACTCCCTCTGCTAGGATATCAGCACTAAACATCGTGGGGGATCTCTTACGGAAAGTAGGGGCTTTAGAATCCAAATTAGCAGCTTGCAGGAATTTTGCAAAGGACCAAGCATCACGAAAATCCTATATTTCAGGGAATGTTAACTGTGGGGTGCTGAATGGCAATGGCACAAAGTTCTCTCGATCAGGGCATACATCTTTCTTCGACAAAGGGCAAGAAAAAGTCATATTTCCCACGTTGTTCATGGGGCAGTAA Translation Sequence: MDGEDIPDFS SLKEETAYWK ELSLKYKQSF QEARDELVEF QEGSRELEAE LEAQLVQAEQ RNRDLQADNQ RLKYEVEALK EKLEHQYAQS YKQVSVLEDD LSQTRAIKEQ LHKYVRELEQ ANDDLERAKR ATIVSLEDFE QRLNQAIERN AFLESELDEK ESLLVSVQRL KDEARDLRQE LAVRERQQEV TRKSAPSSPT LDCEKMDSAV QASLSLPATP VGKGTENTFP SPKAIPNGFG TSPLTPSARI SALNIVGDLL RKVGALESKL AACRNFAKDQ ASRKSYISGN VNCGVLNGNG TKFSRSGHTS FFDKGQEKVI FPTLFMGQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
