Ribosomes are composed of 60S and 40S subunits that are assembled in the nucleolus and exported to the cytoplasm through nuclear pore complexes in the nuclear envelope. NMD3 is an adaptor for 60S subunit export via the CRM1 (XPO1, MIM 602559) pathway. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1512bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGAGTATATGGCAGAATCCACCGACCGCAGCCCTGGACACATCTTGTGCTGTGAGTGTGGTGTTCCGATAAGTCCAAATCCTGCCAATATTTGTGTGGCCTGTTTGCGAAGTAAAGTGGACATCAGCCAAGGTATTCCGAAACAAGTCTCGATTTCGTTCTGCAAACAATGTCAAAGGTATTTTCAACCACCAGGAACTTGGATACAGTGTGCTTTAGAATCCAGGGAACTTCTTGCTTTGTGCTTGAAAAAAATCAAAGCCCCTCTGAGTAAGGTACGGCTTGTAGATGCAGGCTTTGTTTGGACTGAGCCTCATTCTAAGAGACTTAAAGTTAAACTGACTATTCAGAAAGAGGTGATGAATGGTGCTATCCTTCAACAAGTGTTTGTGGTGGATTATGTTGTTCAGTCCCAAATGTGTGGAGATTGCCATAGAGTAGAAGCTAAGGATTTCTGGAAGGCTGTGATTCAAGTGAGGCAAAAGACTTTGCATAAAAAAACTTTCTACTATCTGGAACAGTTAATTCTGAAATATGGAATGCATCAGAATACACTTCGTATCAAAGAGATTCATGATGGTCTGGATTTTTATTATTCCTCAAAACAACATGCTCAGAAGATGGTCGAATTTCTTCAGTGTACAGTTCCCTGTAGATACAAAGCATCACAAAGACTGATCTCTCAAGATATCCATAGTAACACATACAATTACAAAAGCACTTTTTCTGTGGAAATTGTTCCAATATGCAAGGATAATGTTGTCTGTCTGTCTCCAAAACTGGCACAAAGCCTGGGAAATATGAACCAGATTTGTGTGTGTATTCGAGTAACCAGTGCCATTCACCTCATTGATCCAAACACCCTACAAGTGGCAGATATTGATGGGAGCACTTTCTGGAGTCACCCTTTCAATAGTTTATGTCATCCCAAACAGCTAGAGGAGTTTATTGTGATGGAATGCAGCATAGTCCAAGATATAAAACGTGCTGCAGGTGCTGGAATGATATCAAAAAAGCATACCCTCGGGGAAGTCTGGGTACAGAAGACATCTGAAATGAATACAGATAAACAGTATTTTTGTCGTACTCATTTGGGACATCTTCTAAATCCCGGAGACCTGGTGTTAGGGTTTGATTTGGCCAACTGTAACTTAAATGATGAGCATGTCAACAAAATGAACTCAGATAGAGTTCCAGATGTGGTATTAATCAAGAAGAGCTATGACCGGACCAAACGTCAGCGTCGTAGAAACTGGAAATTGAAAGAGCTTGCAAGAGAGAGAGAAAACATGGATACAGATGATGAAAGGCAATACCAAGATTTTCTTGAAGATCTTGAAGAAGATGAGGCAATTCGAAAAAATGTCAACATTTACAGAGATTCAGCCATCCCTGTGGAAAGTGACACCGATGATGAAGGAGCACCTCGAATTAGTCTGGCTGAGATGCTTGAAGACCTTCATATTTCCCAAGATGCCACTGGTGAAGAAGGTGCATCAATGCTGACATAA Translation Sequence: MEYMAESTDR SPGHILCCEC GVPISPNPAN ICVACLRSKV DISQGIPKQV SISFCKQCQRYFQPPGTWIQ CALESRELLA LCLKKIKAPL SKVRLVDAGF VWTEPHSKRL KVKLTIQKEVMNGAILQQVF VVDYVVQSQM CGDCHRVEAK DFWKAVIQVR QKTLHKKTFY YLEQLILKYGMHQNTLRIKE IHDGLDFYYS SKQHAQKMVE FLQCTVPCRY KASQRLISQD IHSNTYNYKSTFSVEIVPIC KDNVVCLSPK LAQSLGNMNQ ICVCIRVTSA IHLIDPNTLQ VADIDGSTFWSHPFNSLCHP KQLEEFIVME CSIVQDIKRA AGAGMISKKH TLGEVWVQKT SEMNTDKQYFCRTHLGHLLN PGDLVLGFDL ANCNLNDEHV NKMNSDRVPD VVLIKKSYDR TKRQRRRNWKLKELAREREN MDTDDERQYQ DFLEDLEEDE AIRKNVNIYR DSAIPVESDT DDEGAPRISLAEMLEDLHIS QDATGEEGAS MLT Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
