NOSIP encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 906bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGACGCGGCATGGCAAGAACTGCACCGCAGGGGCCGTCTACACCTACCACGAGAAGAAGAAGGACACAGCGGCCTCGGGCTATGGGACCCAGAACATTCGACTGAGCCGGGATGCCGTGAAGGACTTCGACTGCTGTTGTCTCTCCCTGCAGCCTTGCCACGATCCTGTTGTCACCCCAGATGGCTACCTGTATGAGCGTGAGGCCATCCTGGAGTACATTCTGCACCAGAAGAAGGAGATTGCCCGGCAGATGAAGGCCTACGAGAAGCAGCGGGGCACCCGGCGCGAGGAGCAGAAGGAGCTTCAGCGGGCGGCCTCGCAGGACCATGTGCGGGGCTTCCTGGAGAAGGAGTCGGCTATCGTGAGCCGGCCCCTCAACCCTTTCACAGCCAAGGCCCTCTCGGGCACCAGCCCAGATGATGTCCAACCTGGGCCCAGTGTGGGTCCTCCAAGTAAGGACAAGGACAAAGTGCTGCCCAGCTTCTGGATCCCGTCGCTGACGCCCGAAGCCAAGGCCACCAAGCTGGAGAAGCCGTCCCGCACGGTGACCTGCCCCATGTCAGGGAAGCCCCTGCGCATGTCGGACCTGACGCCCGTGCACTTCACACCGCTAGACAGCTCCGTGGACCGCGTGGGGCTCATCACCCGCAGCGAGCGCTACGTGTGTGCCGTGACCCGCGACAGCCTGAGCAACGCCACCCCCTGCGCTGTGCTGCGGCCCTCTGGGGCTGTGGTCACCCTCGAATGCGTGGAGAAGCTGATTCGGAAGGACATGGTGGACCCTGTGACTGGAGACAAACTCACAGACCGCGACATCATCGTGCTGCAGCGGGGCGGTACCGGCTTCGCGGGCTCCGGAGTGAAGCTGCAAGCGGAGAAATCACGGCCGGTGATGCAGGCCTGA Translation Sequence: MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL QPCHDPVVTPDGYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK ELQRAASQDH VRGFLEKESAIVSRPLNPFT AKALSGTSPD DVQPGPSVGP PSKDKDKVLP SFWIPSLTPE AKATKLEKPSRTVTCPMSGK PLRMSDLTPV HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLRPSGAVVTLEC VEKLIRKDMV DPVTGDKLTD RDIIVLQRGG TGFAGSGVKL QAEKSRPVMQA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
