PDRG1, also known as PDRG or C20orf126, is a 133aa protein that localizes to the cytoplasm and belongs to the prefoldin subunit beta family. Expressed predominately in normal testicular tissue, PDRG1 is functionally induced by ultraviolet light and is thought to play a role in chaperone-mediated protein folding, possibly playing a role in cellular degradation. It is associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 402bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCTATCAC CCGAGGCAGA GCGAGTGCTG CGGTACCTTG TAGAAGTGGA GGAGCTCGCC GAGGAGGTGC TGGCGGACAA GCGGCAGATT GTGGACCTGG ACACTAAAAG GAATCAGAAT CGAGAGGGCC TGAGGGCCCT GCAGAAGGAT CTCAGCCTCT CTGAAGATGT GATGGTTTGC TTCGGGAACA TGTTTATCAA GATGCCTCAC CCTGAGACAA AGGAAATGAT TGAAAAAGAT CAAGATCATC TGGATAAAGA AATAGAAAAA CTGCGGAAGC AACTTAAAGT GAAGGTCAAC CGCCTTTTTG AGGCCCAAGG CAAACCGGAG CTGAAGGGTT TTAACTTGAA CCCCCTCAAC CAGGATGAGC TTAAAGCTCT CAAGGTCATC TTGAAAGGAT GA Translation Sequence: MLSPEAERVL RYLVEVEELA EEVLADKRQI VDLDTKRNQN REGLRALQKD LSLSEDVMVC FGNMFIKMPH PETKEMIEKD QDHLDKEIEK LRKQLKVKVN RLFEAQGKPE LKGFNLNPLN QDELKALKVI LKG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
