Pin1, Clone: [3G8], Mouse, Monoclonal

Product Overview

• Article Name: Pin1, Clone: [3G8], Mouse, Monoclonal
• Biozol Catalog Number: USB-552168
• Supplier Catalog Number: 552168
• Alternative Catalog Number: USB-552168-50,USB-552168-100
• Manufacturer: US Biological
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Immunogen: Recombinant protein corresponding to aa1-163 from human Pin1, expressed in E. coli.

Product Description

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLk I, Wee, and Myt1 kinases), several transcription factors likebeta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [3G8]
• NCBI: 006212
• UniProt: Q13526
• Purity: Purified by Protein A affinity chromatography from tissue culture supernatant..
• Form: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol