PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 471bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCCCATGG CGGGGCTTCT AAAGGGGCTT GTACGGCAAC TGGAGCAGTT CAGAGTTCAA CAACAAGCTT CCAAGATGCC GCCCAAAGGA AAAAGTGGTT CTGGAAAAGC GGGGAAAGGG GGAGCAGCCT CTGGGAGTGA CAGTGCTGAC AAGAAGGCTC AAGGTCCCAA AGGTGGTGGC AATGCAGTAA AGGTCAGACA CATTCTATGT GAAAAACATG GCAAAATCAT GGAAGCCATG GAAAAGTTAA AGTCTGGGAT GAGATTCAAT GAAGTGGCCG CACAGTATAG TGAAGATAAA GCCAGGCAAG GGGGTGACTT GGGTTGGATG ACCAGAGGGT CCATGGTGGG ACCATTTCAA GAAGCAGCAT TTGCCTTGCC TGTAAGTGGG ATGGATAAGC CTGTGTTTAC AGACCCACCG GTTAAGACAA AATTTGGATA TCATATTATT ATGGTCGAAG GAAGAAAATA A Translation Sequence: MPMAGLLKGL VRQLEQFRVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
