PLEKHA3 (Pleckstrin homology domain-containing family A member 3) is involved in Golgi to cell surface membrane traffic and induces membrane tubulation. It binds specifically to phosphatidylinositol-4-phosphate (PtdIns4P), but not to other phosphoinositides. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 903bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGAGGGGGTGTTGTACAAGTGGACCAACTATCTCACAGGCTGGCAGCCTCGTTGGTTTGTTTTAGATAATGGAATCTTATCCTACTATGATTCACAAGATGATGTTTGCAAAGGGAGCAAAGGAAGCATAAAGATGGCAGTTTGTGAAATTAAAGTTCATTCAGCAGACAACACAAGAATGGAATTAATCATTCCTGGAGAGCAGCATTTCTACATGAAGGCAGTGAATGCAGCTGAAAGACAGAGGTGGCTGGTCGCTCTGGGGAGCTCCAAAGCATGTTTGACTGATACAAGGACTAAAAAAGAAAAAGAAATAAGTGAAACCAGTGAATCGCTGAAAACCAAAATGTCTGAACTTCGCCTCTACTGTGACCTCTTAATGCAGCAAGTTCATACAATACAGGAATTTGTTCACCATGATGAGAATCATTCATCTCCTAGTGCAGAGAACATGAATGAAGCCTCTTCTCTGCTTAGTGCCACGTGTAACACATTCATCACAACGCTTGAGGAATGTGTGAAGATAGCCAATGCCAAGTTTAAACCTGAGATGTTTCAACTGCACCATCCGGATCCCTTAGTTTCTCCTGTGTCACCTTCTCCTGTTCAAATGATGAAGCGTTCTGTCAGCCACCCTGGTTCTTGCAGTTCAGAGAGGAGTAGCCACTCTATAAAAGAACCAGTATCTACACTTCACCGACTCTCCCAGCGACGCCGAAGAACCTACTCAGATACAGATTCTTGTAGTGATATTCCTCTTGAAGACCCAGATAGACCTGTTCACTGTTCAAAAAATACACTTAATGGAGATTTGGCATCAGCAACCATTCCTGAAGAAAGCAGACTTATGGCCAAAAAACAATCTGAATCAGAAGATACTCTTCCATCCTTCTCTTCCTGA Translation Sequence: MEGVLYKWTN YLTGWQPRWF VLDNGILSYY DSQDDVCKGS KGSIKMAVCE IKVHSADNTRMELIIPGEQH FYMKAVNAAE RQRWLVALGS SKACLTDTRT KKEKEISETS ESLKTKMSELRLYCDLLMQQ VHTIQEFVHH DENHSSPSAE NMNEASSLLS ATCNTFITTL EECVKIANAKFKPEMFQLHH PDPLVSPVSP SPVQMMKRSV SHPGSCSSER SSHSIKEPVS TLHRLSQRRRRTYSDTDSCS DIPLEDPDRP VHCSKNTLNG DLASATIPEE SRLMAKKQSE SEDTLPSFSS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
