PLEKHO1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). It may function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1230bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGATGAAGAAGAACAATTCCGCCAAGCGGGGACCTCAGGATGGAAACCAGCAGCCTGCACCGCCCGAGAAGGTCGGCTGGGTCCGGAAATTCTGCGGGAAAGGGATTTTCAGGGAGATTTGGAAAAACCGCTATGTGGTGCTGAAAGGGGACCAGCTCTACATCTCTGAGAAGGAGGTAAAAGATGAGAAAAATATTCAAGAGGTATTTGACCTGAGTGACTATGAGAAGTGTGAAGAGCTCCGGAAGTCCAAGAGCAGGAGCAAGAAAAATCATAGCAAGTTTACTCTTGCCCACTCCAAACAGCCCGGTAACACGGCACCCAACCTGATCTTCCTGGCAGTGAGTCCAGAAGAGAAGGAATCGTGGATCAATGCCCTCAACTCTGCCATCACCCGAGCCAAGAACCGTATCTTGGATGAGGTCACCGTTGAGGAGGACAGCTATCTTGCCCATCCCACTCGAGACAGGGCAAAAATCCAGCACTCCCGCCGCCCCCCAACAAGGGGACACCTAATGGCTGTGGCTTCCACCTCTACCTCGGATGGGATGCTGACCTTGGACTTGATCCAAGAGGAAGACCCTTCCCCTGAGGAACCAACCTCTTGTGCTGAGAGCTTTCGGGTTGACCTGGACAAGTCTGTGGCCCAGCTGGCAGGGAGCCGGCGGAGAGCGGACTCAGACCGCATCCAGCCCTCCGCAGACCGGGCAAGCAGTCTCTCCCGACCTTGGGAAAAAACAGACAAAGGGGCCACCTACACCCCCCAGGCACCCAAGAAGTTGACGCCCACAGAGAAAGGCCGCTGCGCCTCCCTGGAGGAGATCCTATCTCAGCGGGATGCTGCCTCTGCCCGCACCCTCCAGCTGCGGGCTGAGGAACCCCCAACCCCTGCCCTCCCCAACCCGGGGCAGCTGTCCCGGATCCAGGACCTGGTAGCAAGGAAACTGGAGGAGACTCAGGAGCTTCTGGCAGAGGTTCAGGGACTGGGAGATGGGAAGCGAAAGGCCAAGGACCCCCCTCGGTCTCCGCCGGATTCTGAGTCAGAGCAGCTGCTGCTGGAGACGGAACGGCTGCTGGGAGAGGCATCATCGAATTGGAGCCAGGCAAAGAGGGTGCTGCAGGAGGTCAGGGAGCTGAGAGACCTGTACAGACAGATGGACCTGCAGACCCCGGACTCCCACCTCAGACAGACCACCCCGCACAGTCAGTACCGGAAGAGCCTGATGTGA Translation Sequence: MMKKNNSAKR GPQDGNQQPA PPEKVGWVRK FCGKGIFREI WKNRYVVLKG DQLYISEKEVKDEKNIQEVF DLSDYEKCEE LRKSKSRSKK NHSKFTLAHS KQPGNTAPNL IFLAVSPEEKESWINALNSA ITRAKNRILD EVTVEEDSYL AHPTRDRAKI QHSRRPPTRG HLMAVASTSTSDGMLTLDLI QEEDPSPEEP TSCAESFRVD LDKSVAQLAG SRRRADSDRI QPSADRASSLSRPWEKTDKG ATYTPQAPKK LTPTEKGRCA SLEEILSQRD AASARTLQLR AEEPPTPALPNPGQLSRIQD LVARKLEETQ ELLAEVQGLG DGKRKAKDPP RSPPDSESEQ LLLETERLLGEASSNWSQAK RVLQEVRELR DLYRQMDLQT PDSHLRQTTP HSQYRKSLM Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
