Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to BamH I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 789bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCAGTCACCGCCCAGGCAGCCCGCAGGAAGGAGCGCGTCCTCTGCCTGTTTGACGTGGACGGGACCCTCACGCCGGCTCGCCAGAAAATTGACCCTGAGGTGGCCGCCTTCCTGCAGAAGCTACGAAGTAGAGTGCAGATCGGTGTGGTGGGCGGCTCTGACTACTGTAAGATCGCTGAGCAGCTGGGTGACGGGGATGAAGTCATTGAGAAGTTTGATTATGTGTTTGCCGAGAACGGGACGGTGCAGTATAAGCACGGACGACTGCTCTCCAAGCAGACCATCCAGAACCACCTGGGGGAGGAGCTGCTGCAGGACTTGATCAACTTCTGCCTCAGCTACATGGCCCTGCTCAGGCTGCCCAAGAAGCGTGGAACCTTCATCGAGTTCCGGAATGGCATGCTGAACATCTCGCCCATCGGCCGGAGCTGCACCCTGGAGGAGAGGATCGAGTTCTCCGAACTGGACAAGAAAGAGAAGATCCGGGAGAAGTTCGTGGAAGCCCTGAAAACAGAGTTTGCTGGCAAAGGGCTGAGGTTCTCTCGAGGAGGCATGATCAGCTTTGACGTCTTCCCCGAGGGCTGGGACAAGCGCTACTGCCTGGATAGCCTGGACCAGGACAGCTTCGACACCATCCACTTCTTTGGGAACGAGACTAGCCCTGGTGGGAACGACTTTGAGATCTTTGCCGACCCCCGGACTGTTGGCCACAGCGTGGTGTCTCCTCAGGACACGGTGCAGCGATGCCGGGAGATTTTCTTCCCAGAGACAGCTCATGAGGCGTGA Translation Sequence: MAVTAQAARR KERVLCLFDV DGTLTPARQK IDPEVAAFLQ KLRSRVQIGV VGGSDYCKIA EQLGDGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNISP IGRSCTLEER IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY CLDSLDQDSF DTIHFFGNET SPGGNDFEIF ADPRTVGHSV VSPQDTVQRC REIFFPETAH EA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
