PSTK (Phosphoseryl-TRNA Kinase) is specifically phosphorylates seryl-tRNA (Sec) to O-phosphoseryl-tRNA (Sec), an activated intermediate for selenocysteine biosynthesis Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1047bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAAGACCGCCGAGAACATCAGAGGAACCGGCAGCGACGGGCCGCGGAAACGAGGCCTCTGCGTCCTCTGTGGCCTCCCCGCGGCAGGAAAATCGACTTTCGCGCGCGCCCTCGCCCACCGGCTGCAGCAGGAGCAGGGTTGGGCCATCGGTGTTGTCGCGTATGATGACGTCATGCCCGACGCGTTTCTCGCCGGGGCAAGAGCGCGACCGGCGCCATCCCAATGGAAATTGCTTCGACAGGAACTGTTGAAGTACCTGGAATACTTCTTGATGGCTGTCATTAATGGGTGTCAGATGTCTGTCCCACCCAACAGGACTGAAGCCATGTGGGAAGATTTTATAACCTGCTTAAAGGATCAAGATCTGATATTTTCTGCAGCATTTGAGGCCCAGTCTTGCTACCTCTTAACAAAAACTGCTGTTTCTAGACCTTTGTTTTTGGTTTTGGATGACAATTTTTATTATCAGAGTATGAGATATGAAGTCTACCAGCTGGCTCGGAAATATTCATTGGGCTTTTGCCAGCTCTTTTTAGATTGTCCTCTTGAGACCTGTTTACAGAGGAATGGCCAGAGGCCACAGGCACTGCCTCCTGAGACCATCCACCTGATGCGAAGAAAGCTAGAAAAGCCCAACCCTGAGAAAAATGCTTGGGAACACAACAGCCTCACAATTCCGAGTCCAGCATGTGCTTCGGAGGCCAGCCTGGAAGTGACTGATTTATTGCTCACTGCTTTGGAAAATCCAGTAAAATATGCTGAGGACAATATGGAACAAAAGGACACAGACAGAATTATTTGTTCAACTAACATTCTTCATAAAACTGATCAGACACTCCGAAGGATTGTATCTCAGACAATGAAGGAAGCAAAAGGTAATCAGGAAGCCTTCTCAGAGATGACATTTAAGCAAAGATGGGTAAGAGCGAACCATGCAGCTATCTGGAGGATCATTCTAGGCAATGAACACATCAAGTGCAGAAGCGCAAAGGTTGGCTGGTTGCAGTGTTGCAGAATCGAGAAGAGGCCATTGAGCACGGGGTGA Translation Sequence: MKTAENIRGT GSDGPRKRGL CVLCGLPAAG KSTFARALAH RLQQEQGWAI GVVAYDDVMP DAFLAGARAR PAPSQWKLLR QELLKYLEYF LMAVINGCQM SVPPNRTEAM WEDFITCLKD QDLIFSAAFE AQSCYLLTKT AVSRPLFLVL DDNFYYQSMR YEVYQLARKY SLGFCQLFLD CPLETCLQRN GQRPQALPPE TIHLMRRKLE KPNPEKNAWE HNSLTIPSPA CASEASLEVT DLLLTALENP VKYAEDNMEQ KDTDRIICST NILHKTDQTL RRIVSQTMKE AKGNQEAFSE MTFKQRWVRA NHAAIWRIIL GNEHIKCRSA KVGWLQCCRI EKRPLSTG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
