RNF114 contains 1 RING-type zinc finger. The protein is expressed in numerous tissues, including skin, CD4 lymphocytes and dendritic cells. It may play a role in spermatogenesis. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 687bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCGGCGC AACAGCGGGA CTGCGGGGGT GCTGCGCAGC TGGCGGGGCC GGCGGCGGAG GCTGACCCCC TAGGACGCTT CACGTGTCCC GTGTGCTTAG AGGTGTACGA GAAGCCGGTA CAGGTGCCCT GCGGACACGT CTTTTGCTCT GCATGCCTGC AGGAATGTCT GAAGCCGAAG AAGCCTGTCT GTGGGGTGTG TCGCAGCGCT CTGGCACCTG GCGTCCGAGC CGTGGAGCTC GAGCGGCAGA TCGAGAGCAC AGAGACTTCT TGCCATGGCT GCCGTAAGAA TTTCTTCCTG TCCAAGATCC GGTCCCACGT GGCTACTTGT TCCAAATACC AGAATTACAT CATGGAAGGT GTGAAGGCCA CCATTAAGGA TGCATCTCTT CAGCCAAGGA ATGTTCCAAA CCGTTACACC TTTCCTTGTC CTTACTGTCC TGAGAAGAAC TTTGATCAGG AAGGACTTGT GGAACACTGC AAATTATTCC ATAGCACGGA TACCAAATCT GTGGTTTGTC CGATATGTGC CTCGATGCCC TGGGGAGACC CCAACTACCG CAGCGCCAAC TTCAGAGAGC ACATCCAGCG CCGGCACCGG TTTTCTTATG ACACTTTTGT GGATTATGAT GTTGATGAAG AGGACATGAT GAATCAGGTG TTGCAGCGCT CCATCATCGA CCAGTGA Translation Sequence: MAAQQRDCGG AAQLAGPAAE ADPLGRFTCP VCLEVYEKPV QVPCGHVFCS ACLQECLKPK KPVCGVCRSA LAPGVRAVEL ERQIESTETS CHGCRKNFFL SKIRSHVATC SKYQNYIMEG VKATIKDASL QPRNVPNRYT FPCPYCPEKN FDQEGLVEHC KLFHSTDTKS VVCPICASMP WGDPNYRSAN FREHIQRRHR FSYDTFVDYD VDEEDMMNQV LQRSIIDQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
