SH3GLB2 (SH3-Domain GRB2-Like Endophilin B2) is a Protein Coding gene. Among its related pathways are Endocytosis. An important paralog of this gene is SH3GL3. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1188bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGACTTCAACATGAAGAAGCTGGCGTCGGACGCGGGCATCTTCTTCACCCGGGCGGTGCAGTTCACGGAGGAGAAATTTGGCCAGGCTGAGAAGACTGAGCTTGATGCCCACTTTGAAAACCTTCTGGCCCGGGCAGACAGCACCAAGAACTGGACAGAGAAGATCTTGAGGCAGACAGAGGTGCTGCTGCAGCCCAACCCCAGTGCCCGAGTGGAGGAGTTCCTGTATGAGAAGCTGGACAGGAAGGTCCCCTCAAGGGTCACCAACGGGGAGCTGCTGGCTCAGTACATGGCAGACGCGGCCAGTGAGCTGGGGCCGACCACCCCCTATGGGAAGACACTGATCAAGGTGGCAGAAGCTGAAAAGCAACTGGGAGCCGCGGAGAGGGATTTTATCCACACGGCCTCCATCAGCTTCCTCACACCCTTGCGCAACTTCCTGGAGGGGGACTGGAAGACCATCTCGAAGGAGAGGCGGCTCCTCCAAAACCGGCGTCTGGACTTGGATGCCTGCAAAGCGAGGCTGAAGAAGGCCAAGGCTGCAGAAGCCAAAGCCACGACGGTGCCTGACTTTCAGGAGACTAGACCTCGTAATTACATTCTCTCGGCCAGCGCCTCCGCGCTCTGGAATGATGAAGTGGACAAGGCCGAGCAGGAGCTCCGCGTGGCCCAGACAGAGTTTGACCGGCAAGCAGAAGTGACCCGTCTCTTGCTGGAGGGAATCAGTAGCACTCACGTGAACCACCTGCGCTGCCTCCACGAGTTCGTCAAGTCTCAGACAACCTACTACGCACAGTGCTACCGCCACATGCTGGACTTGCAGAAGCAGCTGGGCAGATTTCCCGGCACCTTCGTGGGCACCACAGAGCCCGCCTCCCCACCCCTGAGCAGCACCTCACCCACCACTGCTGCGGCCACTATGCCTGTGGTGCCCTCTGTGGCCAGCCTGGCCCCTCCGGGGGAGGCCTCGCTCTGCCTGGAAGAGGTGGCCCCCCCTGCCAGTGGGACCCGCAAAGCTCGGGTGCTCTATGACTACGAGGCAGCCGACAGCAGTGAGCTGGCCCTGCTGGCTGATGAGCTCATCACTGTCTACAGCCTGCCTGGCATGGACCCTGACTGGCTCATTGGCGAGAGAGGCAACAAGAAGGGCAAGGTCCCTGTCACCTACTTGGAACTGCTCAGCTAG Translation Sequence: MDFNMKKLAS DAGIFFTRAV QFTEEKFGQA EKTELDAHFE NLLARADSTK NWTEKILRQT EVLLQPNPSA RVEEFLYEKL DRKVPSRVTN GELLAQYMAD AASELGPTTP YGKTLIKVAE AEKQLGAAER DFIHTASISF LTPLRNFLEG DWKTISKERR LLQNRRLDLD ACKARLKKAK AAEAKATTVP DFQETRPRNY ILSASASALW NDEVDKAEQE LRVAQTEFDR QAEVTRLLLE GISSTHVNHL RCLHEFVKSQ TTYYAQCYRH MLDLQKQLGR FPGTFVGTTE PASPPLSSTS PTTAAATMPV VPSVASLAPP GEASLCLEEV APPASGTRKA RVLYDYEAAD SSELALLADE LITVYSLPGM DPDWLIGERG NKKGKVPVTY LELLS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
