SIRT2 (NAD-dependent deacetylase sirtuin-2) is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIRT2 is a NAD-dependent deacetylase, which deacetylates the Lys-40 of alpha-tubulin. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1059bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGACTTCC TGCGGAACTT ATTCTCCCAG ACGCTCAGCC TGGGCAGCCA GAAGGAGCGT CTGCTGGACG AGCTGACCTT GGAAGGGGTG GCCCGGTACA TGCAGAGCGA ACGCTGTCGC AGAGTCATCT GTTTGGTGGG AGCTGGAATC TCCACATCCG CAGGCATCCC CGACTTTCGC TCTCCATCCA CCGGCCTCTA TGACAACCTA GAGAAGTACC ATCTTCCCTA CCCAGAGGCC ATCTTTGAGA TCAGCTATTT CAAGAAACAT CCGGAACCCT TCTTCGCCCT CGCCAAGGAA CTCTATCCTG GGCAGTTCAA GCCAACCATC TGTCACTACT TCATGCGCCT GCTGAAGGAC AAGGGGCTAC TCCTGCGCTG CTACACGCAG AACATAGATA CCCTGGAGCG AATAGCCGGG CTGGAACAGG AGGACTTGGT GGAGGCGCAC GGCACCTTCT ACACATCACA CTGCGTCAGC GCCAGCTGCC GGCACGAATA CCCGCTAAGC TGGATGAAAG AGAAGATCTT CTCTGAGGTG ACGCCCAAGT GTGAAGACTG TCAGAGCCTG GTGAAGCCTG ATATCGTCTT TTTTGGTGAG AGCCTCCCAG CGCGTTTCTT CTCCTGTATG CAGTCAGACT TCCTGAAGGT GGACCTCCTC CTGGTCATGG GTACCTCCTT GCAGGTGCAG CCCTTTGCCT CCCTCATCAG CAAGGCACCC CTCTCCACCC CTCGCCTGCT CATCAACAAG GAGAAAGCTG GCCAGTCGGA CCCTTTCCTG GGGATGATTA TGGGCCTCGG AGGAGGCATG GACTTTGACT CCAAGAAGGC CTACAGGGAC GTGGCCTGGC TGGGTGAATG CGACCAGGGC TGCCTGGCCC TTGCTGAGCT CCTTGGATGG AAGAAGGAGC TGGAGGACCT TGTCCGGAGG GAGCACGCCA GCATAGATGC CCAGTCGGGG GCGGGGGTCC CCAACCCCAG CACTTCAGCT TCCCCCAAGA AGTCCCCGCC ACCTGCCAAG GACGAGGCCA GGACAACAGA GAGGGAGAAA CCCCAGTGA Translation Sequence: MDFLRNLFSQ TLSLGSQKER LLDELTLEGV ARYMQSERCR RVICLVGAGI STSAGIPDFR SPSTGLYDNL EKYHLPYPEA IFEISYFKKH PEPFFALAKE LYPGQFKPTI CHYFMRLLKD KGLLLRCYTQ NIDTLERIAG LEQEDLVEAH GTFYTSHCVS ASCRHEYPLS WMKEKIFSEV TPKCEDCQSL VKPDIVFFGE SLPARFFSCM QSDFLKVDLL LVMGTSLQVQ PFASLISKAP LSTPRLLINK EKAGQSDPFL GMIMGLGGGM DFDSKKAYRD VAWLGECDQG CLALAELLGW KKELEDLVRR EHASIDAQSG AGVPNPSTSA SPKKSPPPAK DEARTTEREK PQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
