SMUG1 is a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene, the full-length nature is known for some but not all of the variants. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 813bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCCCCAGGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCTCATGGAGCCCCAGCCCTGCCCTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCGGCTCAATGCTGAGCTGAGCCAGCTGCAGTTTTCGGAGCCTGTGGGCATCATCTACAATCCCGTGGAGTATGCATGGGAGCCACATCGCAACTACGTGACTCGCTACTGCCAGGGCCCCAAGGAAGTACTCTTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCAGACTGGGGTGCCCTTTGGGGAAGTAAGCATGGTCCGGGACTGGTTGGGCATTGTGGGGCCTGTGCTGACCCCTCCCCAAGAGCATCCTAAACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGTGAGTGGTGCCCGATTCTGGGGCTTTTTCCGGAACCTCTGTGGACAGCCTGAGGTCTTCTTCCATCACTGTTTTGTCCACAATCTATGCCCTCTGCTTTTCCTGGCTCCCAGCGGGCGCAACCTTACTCCTGCTGAGCTGCCTGCCAAGCAGCGAGAACAGCTTCTTGGGATCTGTGATGCAGCCCTCTGCCGGCAGGTGCAGCTGCTGGGGGTGCGGCTGGTGGTGGGAGTTGGGCGACTGGCAGAGCAGCGGGCACGACGGGCTCTGGCAGGCCTGATGCCAGAGGTCCAGGTGGAAGGGCTCCTGCATCCCTCTCCCCGTAACCCACAGGCCAACAAGGGCTGGGAGGCAGTGGCCAAGGAAAGATTGAATGAGCTGGGGCTGCTGCCACTGCTGTTGAAATGA Translation Sequence: MPQAFLLGSI HEPAGALMEP QPCPGSLAES FLEEELRLNA ELSQLQFSEP VGIIYNPVEY AWEPHRNYVT RYCQGPKEVL FLGMNPGPFG MAQTGVPFGE VSMVRDWLGI VGPVLTPPQE HPKRPVLGLE CPQSEVSGAR FWGFFRNLCG QPEVFFHHCF VHNLCPLLFL APSGRNLTPA ELPAKQREQL LGICDAALCR QVQLLGVRLV VGVGRLAEQR ARRALAGLMP EVQVEGLLHP SPRNPQANKG WEAVAKERLN ELGLLPLLLK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
