ST6GALNAC5, Recombinant, Human, aa30-336, His-Tag

Product Overview

• Article Name: ST6GALNAC5, Recombinant, Human, aa30-336, His-Tag
• Biozol Catalog Number: USB-552303
• Supplier Catalog Number: 552303
• Alternative Catalog Number: USB-552303-50,USB-552303-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

ST6GALNAC5, also known as Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5, is a member of the glycosyltransferase 29 family. This protein is a sialyltransferase involved in the biosynthesis of ganglioside GD1a from GM1b. It is involved in the pathway protein glycosylation, which is part of Protein modification. Its expression is restricted to the brain normally. It has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier. Recombinant human ST6GALNAC5, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa30-336 from human ST6GALNAC5, fused His-Tag at C-terminal, was expressed in baculovirus. Amino Acid Sequence: GGQKERP PQQQQQQQQQ QQQASATGSS QPAAESSTQQ RPGVPAGPRP LDGYLGVADH KPLKMHCRDC ALVTSSGHLL HSRQGSQIDQ TECVIRMNDA PTRGYGRDVG NRTSLRVIAH SSIQRILRNR HDLLNVSQGT VFIFWGPSSY MRRDGKGQVY NNLHLLSQVL PRLKAFMITR HKMLQFDELF KQETGKDRKI SNTWLSTGWF TMTIALELCD RINVYGMVPP DFCRDPNHPS VPYHYYEPFG PDECTMYLSH ERGRKGSHHR FITEKRVFKN WARTFNIHFF QPDWKPESLA INHPENKPVF Biological Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range 0.15ng/ml. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 36.4
• NCBI: 112227
• UniProt: Q9BVH7
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol