TIPIN is part of the replisome complex, a group of proteins that support DNA replication. It binds TIM, which is involved in circadian rhythm regulation, and aids in protecting cells against DNA damage and stress. Two pseudogenes and two transcript variants encoding different isoforms have been found for this gene. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 906bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCTAGAACCACAGGAGAATGGCGTGATTGACCTACCAGATTATGAGCATGTAGAAGATGAAACTTTTCCTCCTTTCCCACCTCCAGCCTCTCCAGAGAGACAAGATGGTGAAGGAACTGAGCCTGATGAAGAGTCAGGAAATGGAGCACCTGTTCCTGTACCTCCAAAGAGAACAGTTAAAAGAAATATACCCAAGCTGGATGCTCAGAGATTAATTTCAGAGAGAGGACTTCCAGCCTTAAGGCATGTATTTGATAAGGCAAAATTCAAAGGTAAAGGTCATGAGGCTGAAGACTTGAAGATGCTAATCAGACACATGGAGCACTGGGCACATAGGCTATTCCCTAAACTGCAGTTTGAGGATTTTATTGACAGAGTTGAATACCTGGGAAGTAAAAAGGAAGTTCAGACCTGTTTAAAACGAATTCGACTTGATCTCCCTATTTTACATGAAGATTTTGTTAGCAATAATGATGAAGTTGCGGAGAATAATGAACATGATGTCACTTCTACTGAATTAGATCCCTTTCTGACAAACTTATCTGAAAGTGAGATGTTTGCTTCTGAGTTAAGTAGAAGCCTAACAGAAGAGCAACAACAAAGAATTGAGAGAAATAAACAACTGGCCTTGGAAAGAAGGCAGGCAAAGCTGCTGAGTAATAGTCAGACCCTAGGAAATGATATGTTAATGAATACACCCAGGGCACACACGGTTGAAGAGGTTAATACTGATGAGGATCAAAAGGAGGAGTCAAATGGATTAAACGAAGACATTCTGGACAATCCATGTAATGATGCTATTGCCAATACTTTAAATGAAGAGGAAACACTGCTGGACCAGTCTTTTAAAAATGTGCAACAGCAACTTGATGCTACATCCAGAAATATTACTGAAGCTAGATAA Translation Sequence: MLEPQENGVI DLPDYEHVED ETFPPFPPPA SPERQDGEGT EPDEESGNGA PVPVPPKRTVKRNIPKLDAQ RLISERGLPA LRHVFDKAKF KGKGHEAEDL KMLIRHMEHW AHRLFPKLQFEDFIDRVEYL GSKKEVQTCL KRIRLDLPIL HEDFVSNNDE VAENNEHDVT STELDPFLTNLSESEMFASE LSRSLTEEQQ QRIERNKQLA LERRQAKLLS NSQTLGNDML MNTPRAHTVEEVNTDEDQKE ESNGLNEDIL DNPCNDAIAN TLNEEETLLD QSFKNVQQQL DATSRNITEAR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
