ZNF330 (Zinc Finger Protein 330) is a novel human gene. The deduced protein sequence significantly showed nine highly conserved CXXC motifs indicative of several putative zinc fingers. Immunolocalization of the protein in culture of mammalian cells indicates an association with the interphase nucleolus and a transient relocation to the mitotic centromeres and the midbody. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 963bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGCCTAAAAAAAAGACTGGTGCGAGGAAGAAGGCTGAGAACCGCCGAGAACGTGAAAAACAACTAAGAGCATCAAGAAGCACTATAGATTTAGCTAAACATCCATGTAATGCCTCAATGGAATGTGACAAGTGTCAGAGGCGGCAGAAGAATAGAGCATTTTGCTACTTTTGTAATTCTGTACAGAAGTTACCAATTTGTGCACAGTGTGGGAAAACAAAGTGCATGATGAAGTCTTCAGACTGTGTCATAAAGCATGCTGGTGTATACAGTACTGGCCTTGCAATGGTGGGTGCAATATGTGACTTCTGTGAAGCTTGGGTTTGCCATGGTAGGAAATGTCTCAGTACACATGCTTGTGCCTGCCCTCTTACCGATGCTGAGTGTGTTGAATGTGAACGAGGCGTGTGGGACCATGGAGGCAGAATATTCAGTTGTTCTTTTTGCCATAACTTTCTCTGTGAAGATGATCAATTTGAGCATCAAGCCAGCTGCCAGGTTTTAGAGGCAGAAACATTTAAATGTGTTTCATGCAATCGGCTTGGTCAGCACTCATGTCTCCGTTGTAAGGCTTGTTTCTGTGATGATCATACAAGGAGCAAAGTGTTTAAGCAAGAAAAAGGAAAACAGCCTCCTTGTCCTAAATGTGGGCATGAAACTCAGGAGACTAAGGACCTTAGCATGTCAACACGCTCCCTGAAATTTGGCAGGCAGACTGGAGGTGAAGAGGGAGATGGAGCTTCTGGGTATGATGCTTATTGGAAGAACCTTTCATCTGATAAGTATGGTGATACCAGCTACCACGATGAGGAGGAGGATGAGTATGAAGCAGAGGATGATGAAGAGGAAGAAGATGAAGGCAGAAAGGATTCAGATACTGAGTCATCAGATTTGTTTACTAATTTGAATTTAGGAAGGACCTATGCTAGTGGCTATGCTCACTATGAGGAACAAGAGAACTAG Translation Sequence: MPKKKTGARK KAENRREREK QLRASRSTID LAKHPCNASM ECDKCQRRQK NRAFCYFCNS VQKLPICAQC GKTKCMMKSS DCVIKHAGVY STGLAMVGAI CDFCEAWVCH GRKCLSTHAC ACPLTDAECV ECERGVWDHG GRIFSCSFCH NFLCEDDQFE HQASCQVLEA ETFKCVSCNR LGQHSCLRCK ACFCDDHTRS KVFKQEKGKQ PPCPKCGHET QETKDLSMST RSLKFGRQTG GEEGDGASGY DAYWKNLSSD KYGDTSYHDE EEDEYEAEDD EEEEDEGRKD SDTESSDLFT NLNLGRTYAS GYAHYEEQEN Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
