Acid Ceramidase, Recombinant, Pan Troglodytes, aa22-395, His-Tag, Myc-Tag

Product Overview

• Article Name: Acid Ceramidase, Recombinant, Pan Troglodytes, aa22-395, His-Tag, Myc-Tag
• Biozol Catalog Number: USB-583488
• Supplier Catalog Number: 583488
• Alternative Catalog Number: USB-583488-20, USB-583488-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Source: Recombinant protein corresponding to aa22-395 from Pan troglodytes Acid ceramidase, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~47.7kD Amino Acid Sequence: QHAPPWTEDCRKSTYPPSGPTYRGPVPWYTINLDLPPYKRWHELMLDKAPMLKVIVNSLKNMINTFVPSGKIVQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLSLNERFSINGGYLGILEWILGKKDAMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 47.7
• UniProt: A5A6P2
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.