Adenine Phosphoribosyltransferase, Recombinant, E. coli O139:H28, aa1-183, His-Tag, Myc-Tag

Product Overview

• Article Name: Adenine Phosphoribosyltransferase, Recombinant, E. coli O139:H28, aa1-183, His-Tag, Myc-Tag
• Biozol Catalog Number: USB-583518
• Supplier Catalog Number: 583518
• Alternative Catalog Number: USB-583518-20, USB-583518-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Source: Recombinant protein corresponding to aa1-183 from Escherichia coli O139:H28 Adenine phosphoribosyltransferase, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~27.3kD Amino Acid Sequence: MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALSIDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDLEYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFDLGGEQRLEKQGITSYSLVPFPGH Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 27.3
• UniProt: A7ZIM8
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.