Beta-lactamase, Recombinant, E. coli, aa20-377, His-SUMO-Tag
Biozol Catalog Number:
USB-583789
Supplier Catalog Number:
583789
Alternative Catalog Number:
USB-583789-20,USB-583789-100
Manufacturer:
US Biological
Category:
Molekularbiologie
This protein is a serine beta-lactamase with a substrate specificity for cephalosporins. Source: Recombinant protein corresponding to aa20-377 from Escherichia coli Beta-lactamase, fused to 6X His-SUMO-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~52.5kD Amino Acid Sequence: APQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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