Cobalamin import ATP-binding Protein BtuD, Recombinant, Halobacterium salinarum, aa1-398, His-Tag

Product Overview

• Article Name: Cobalamin import ATP-binding Protein BtuD, Recombinant, Halobacterium salinarum, aa1-398, His-Tag
• Biozol Catalog Number: USB-584026
• Supplier Catalog Number: 584026
• Alternative Catalog Number: USB-584026-20,USB-584026-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably responsible for energy coupling to the transport system. Source: Recombinant protein corresponding to aa1-398 from Halobacterium salinarum Cobalamin import ATP-binding protein BtuD, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~44.9kD Amino Acid Sequence: MTLDVTGLDVELAGTRILDDVHASIRDGHLVGVVGPNGAGKSTLLRAMNGLITPTAGTVLVAGDDVHALSSAAASRRIATVPQDASVSFEFTVRQVVEMGRHPHTTRFGTDTDTAVVDRAMARTGVAQFAARDVTSLSGGERQRVLLARALAQAAPVLLLDEPTASLDVNHQIRTLEVVRDLADSEDRAVVAAIHDLDLAARYCDELVVVADGRVHDAGAPRSVLTPDTIRAAFDARVAVGTDPATGAVTVTPLPDRTSAAADTSVHVVGGGDSATPVVRRLVSAGASVSVGPVVEGDTDHETARRVGCPCTSVAPFTRLEDTTAASATRADIAAADVIAVPVAAAARPGVRGLLTGAVPTLAVGDAAGAPEWADRLVACDAVVSAVGALADTPSDGV Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 44.9
• UniProt: B0R5G4
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.