Colicin-E1, Recombinant, E. coli, aa1-304, His-Tag, Myc-Tag
Biozol Catalog Number:
USB-584036
Supplier Catalog Number:
584036
Alternative Catalog Number:
USB-584036-20,USB-584036-100
Manufacturer:
US Biological
Category:
Molekularbiologie
This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy., Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. Partial recombinant protein corresponding to aa1-304 from Escherichia coli Colicin-E1, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Swiss/Uniprot Accession: P02978 Molecular Weight: ~38.2kD Amino Acid Sequence: METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINAD Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
