Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate. Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC. Source: Recombinant protein corresponding to aa56-533 from Danio rerio D-2-hydroxyglutarate dehydrogenase, mitochondrial, fused to 6X His-SUMO-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~68.3kD Amino Acid Sequence: ANPANPSAAPPRLPFSRVTQEDLSFFRALLPGRTITDPDLLKSSNVDWLKTVQGSSDVLLRPKTTEGVSQILRYCNERNLAVCPQGGNTGLVGGSVPVFDEIILSTSLMNQVFAFDNISGILTCQAGCVLENLSHYLEERDFIMPLDLGAKGSCHIGGNVSTNAGGLRLLRYGSLRGTVLGLEVVLADGHVLNCLATLRKDNTGYDLKQLFIGSEGTLGVITAVSILCPRKPKAVNVAFLGCSSFQQLLETFQCCRGMLGEILSAFEFLDASCMNLLEKHLKLTNPITECPFYIVIETAGSNATHDEEKLHQFLEEVMTSSLVTDGTVATEATKIKALWSLRERVTEALTHEGYTYKYDISLPVEKIYDLVQDMRRHLGGMAKNVVGYGHVGDGNLHLNITSPSKDFDLLAAIEPYVYEWTSQWKGSISAEHGLGLKKRNYIYYSKPSEAVALMGSIKAMLDPKGILNPYKTLPDNIN Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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