DNA Mismatch Repair Protein MutS, Recombinant, Aquifex aeolicus, aa1-859, His-Tag
Biozol Catalog Number:
USB-584272
Supplier Catalog Number:
584272
Alternative Catalog Number:
USB-584272-20,USB-584272-100
Manufacturer:
US Biological
Category:
Molekularbiologie
This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity. Source: Recombinant protein corresponding to aa1-859 from Aquifex aeolicus DNA mismatch repair protein MutS, fused to 6X His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~100.4kD Amino Acid Sequence: MEKSEKELTPMLSQYHYFKNQYPDCLLLFRLGDFYELFYEDAYIGSKELGLVLTSRPAGKGKERIPMCGVPYHSANSYIAKLVNKGYKVAICEQVEDPSKAKGIVKREVVRVITPGTFFERDTGGLASLYKKGNHYYVGYLNLAVGEFLGAKVKIEELLDLLSKLNIKEILVKKGEKLPEELEKVLKVYVSELEEEFFEEGSEEILKDFGVLSLQAFGFEEDTYSLPLGAVYKYAKTTQKGYTPLIPRPKPYRDEGFVRLDIKAIKGLEILESLEGRKDISLFKVIDRTLTGMGRRRLKFRLLSPFRSREKIERIQEGVQELKENREALLKIRQILEGMADLERLVSKISSNMATPRELVYLKNSLKKVEELRLLLLELKAPIFKEILQNFEDTKKIINDIEKTLVEDPPLHVKEGGLIREGVNAYLDELRFIRDNAETYLREYEKKLRQETGIQSLKIGYNKVMGYYIEVTKPNLKYVPSYFRRRQTLSNSERFTTEELQRLEEKILSAQTRINDLEYELYKELRERVVKELDKVGNNASAVAEVDFIQSLAQIAYEKDWAKPQIHEGYELIIEEGRHPVIEEFVENYVPNDTKLDRDSFIHVITGPNMAGKSSYIRQVGVLTLLSHIGSFIPARRAKIPVVDALFTRIGSGDVLALGVSTFMNEMLEVSNILNNATEKSLVILDEVGRGTSTYDGIAISKAIVKYISEKLKAKTLLATHFLEITELEGKIEGVKNYHMEVEKTPEGIRFLYILKEGKAEGSFGIEVAKLAGLPEEVVEEARKILRELEEKENKKEDIVPLLEETFKKSEEAQRLEEYEEIIKKIEEIDIGNTTPLQALLILAELKKKCSFSKKESGA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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