Envelope Glycoprotein B, Recombinant, Human cytomegalovirus, aa552-635, His-Tag, Myc-Tag

Product Overview

• Article Name: Envelope Glycoprotein B, Recombinant, Human cytomegalovirus, aa552-635, His-Tag, Myc-Tag
• Biozol Catalog Number: USB-584398
• Supplier Catalog Number: 584398
• Alternative Catalog Number: USB-584398-20,USB-584398-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. May be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Interacts with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Participates also in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Source: Recombinant protein corresponding to aa552-635 from human cytomegalovirus Envelope glycoprotein B, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~17.1kD Amino Acid Sequence: TINQTSVKVLRDMNVKESPGRCYSRPVVIFNFANSSYVQYGQLGEDNEILLGNHRTEECQLPSLKIFIAGNSAYEYVDYLFKRM Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 17.1
• UniProt: P06473
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.