Eukaryotic Translation Initiation Factor 3 Subunit I, Recombinant, Rat, aa1-325, His-Tag

Product Overview

• Article Name: Eukaryotic Translation Initiation Factor 3 Subunit I, Recombinant, Rat, aa1-325, His-Tag
• Biozol Catalog Number: USB-584472
• Supplier Catalog Number: 584472
• Alternative Catalog Number: USB-584472-20,USB-584472-200
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2. Source: Recombinant protein corresponding to aa1-325 from rat Eukaryotic translation initiation factor 3 subunit I, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~40.5kD Amino Acid Sequence: MKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDADWDTKHVLTGSADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQCFVSFFDLRDPSQIDSNEPYMKIPCNDSKITSAVWGPLGECIIAGHESGELNQYSAKSGEVLVNVKEHSRQINDIQLSRDMTMFVTASKDNTAKLFDSTSLEHQKTFRTERPVNSAALSPNYDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGRVKGHFGPINSVAFHPDGKSYSSGGEDGYVRIHYFDPQYFEFEFEA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 40.5
• UniProt: B0BNA7
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.