HLA Class II Histocompatibility Antigen, DR Alpha Chain, Recombinant, Human, aa26-254, His-Tag

Product Overview

• Article Name: HLA Class II Histocompatibility Antigen, DR Alpha Chain, Recombinant, Human, aa26-254, His-Tag
• Biozol Catalog Number: USB-584873
• Supplier Catalog Number: 584873
• Alternative Catalog Number: USB-584873-20, USB-584873-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. The selection of the immunodominant epitopes follows two processing modes: bind first, cut/trim later for pathogen-derived antigenic peptides and cut first, bind later for autoantigens/self-peptides. The anchor residue at position 1 of the peptide N-terminal, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules. Source: Recombinant protein corresponding to aa26-254 from human HLA class II histocompatibility antigen, DR alpha chain, fused to 6X His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~28.0kD Amino Acid Sequence: IKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENVVCALGLTVGLVGIIIGTIFIIKGVRKSNAAERRGPL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 28
• UniProt: P01903
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.