Large Envelope Protein, Recombinant, Hepatitis B Virus Genotype C Subtype adr, aa2-181, His-Tag

Product Overview

• Article Name: Large Envelope Protein, Recombinant, Hepatitis B Virus Genotype C Subtype adr, aa2-181, His-Tag
• Biozol Catalog Number: USB-585156
• Supplier Catalog Number: 585156
• Alternative Catalog Number: USB-585156-20,USB-585156-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

The large envelope protein exists in two topological conformations, one which is termed external or Le-HBsAg and the other internal or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein., The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid. Source: Recombinant protein corresponding to aa2-181 from Hepatitis B virus genotype C subtype adr Large envelope protein, fused to 6X His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~20.9kD Amino Acid Sequence: GGWSSKPRQGMGTNLSVPNPLGFFPDHQLDPAFGANSNNPDWDFNPNKDHWPEANQVGVGTFGPGFTPPHGGLLGWSPQAQGILTTVPAAPPPASTNRQSGRQPTPISPPLRDSHPQAMQWNSTTFHQALLDPRVRGLYFPAGGSSSGTVNPVPTTASPISSIFSRTGDPAPNMENTTSG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 20.9
• UniProt: P31868
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.