Heterodimer protein that may serve both defensive (pain-inducing) and predatory (insecticidal) roles. Has membrane-disrupting activity and shows induction of non-specific calcium influx into cells,. Shows broad-spectrum activity against a diverse range of bacteria, and cell lines, as well as hemolytic activity (EC(50)=2.18 uM). In vivo, shows moderate insecticidal activity against D.melanogaster and potent anthelmintic activity against the veterinary nematode H.contortus. In addition, intraplantar injection into mice induces nocifensive behavior and mechanical allodynia. Source: Recombinant protein corresponding to aa1-23 from Myrmecia pilosula M-myrmeciitoxin-Mp2b, fused to GST-Tag at N-terminal and 6X His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~30.8kD Amino Acid Sequence: LIGLVSKGTCVLVKTVCKKVLKQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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