Nucleoprotein, Recombinant, Influenza A virus, aa1-498, His-Tag

Product Overview

• Article Name: Nucleoprotein, Recombinant, Influenza A virus, aa1-498, His-Tag
• Biozol Catalog Number: USB-585595
• Supplier Catalog Number: 585595
• Alternative Catalog Number: USB-585595-20, USB-585595-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoproteins nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoproteins nuclear localization signals, targeting the RNP to the nucleus. Source: Recombinant protein corresponding to aa1-498 from Influenza A virus Nucleoprotein, fused to 6X His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~58.2kD Amino Acid Sequence: MASQGTKRPYEQMETGGERQNATEIRASVGKMVGGIGRFYIQMCTELKLNDYEGRLIQNSITIEKMVLSAFDERRNKYLEEHPNTGKDPKKTGGPIYRKREGKWIRELILYDKEEIRRIWRQANNGEDATAGLTHLMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGIGTMVMELIRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLIVASGYDFEREGYSLVGVDPFKLLQNSQIFSLIRPNENPAHKSQLVWMACHSAAFEDLRVSSFIRGTKVIPRGQLSTRGIQIASNENMETIDSNTLELRSRYWAIRTKSGGNTSQQKASAGQISVQPTFSVQRNLPFERTTIMAAFTGNNEGRTSDMRTEIIRMMENAKPDDVSFQGRGVFELSDEKATNPIVPSFDMSKEGSYFFGDNAEEFDN Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 58.2
• UniProt: P16980
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.