Outer Capsid Protein Lambda-2, Recombinant, Reovirus type 3, aa1-200, His-Tag, Myc-Tag

Product Overview

• Article Name: Outer Capsid Protein Lambda-2, Recombinant, Reovirus type 3, aa1-200, His-Tag, Myc-Tag
• Biozol Catalog Number: USB-585659
• Supplier Catalog Number: 585659
• Alternative Catalog Number: USB-585659-20,USB-585659-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5 cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2O-methyltransferase activities. Source: Recombinant protein corresponding to aa1-200 from Reovirus type 3 Outer capsid protein lambda-2, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~30.2kD Amino Acid Sequence: MANVWGVRLADSLSSPTIETRTRQYTLHDLCSDLDANPGREPWKPLRNQRTNNIVAVQLFRPLQGLVLDTQLYGFPGAFDDWERFMREKLRVLKYEVLRIYPISNYSNEHVNVFVANALVGAFLSNQAFYDLLPLLIINDTMIGDLLGTGASLSQFFQSHGDVLEVAAGRKYLQMENYSNDDDDPPLFAKDLSDYAKAFY Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 30.2
• UniProt: P11079
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.