Protein E6, Recombinant, Human Papillomavirus Type 11, aa1-150, His-Tag

Product Overview

• Article Name: Protein E6, Recombinant, Human Papillomavirus Type 11, aa1-150, His-Tag
• Biozol Catalog Number: USB-585962
• Supplier Catalog Number: 585962
• Alternative Catalog Number: USB-585962-20,USB-585962-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Plays a major role in the induction and maintenance of cellular transformation. E6 associates with host UBE3A/E6-AP ubiquitin-protein ligase and modulates its activity. Sequesters tumor suppressor TP53 in the host cytoplasm and modulates its activity by interacting with host EP300 that results in the reduction of TP53 acetylation and activation. In turn, apoptosis induced by DNA damage is inhibited. E6 protects also host keratinocytes from apoptosis by mediating the degradation of host BAK1. May also inhibit host immune response. Source: Recombinant protein corresponding to aa1-150 from human papillomavirus type 11 Protein E6, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~23.4kD Amino Acid Sequence: MESKDASTSATSIDQLCKTFNLSLHTLQIQCVFCRNALTTAEIYAYAYKNLKVVWRDNFPFAACACCLELQGKINQYRHFNYAAYAPTVEEETNEDILKVLIRCYLCHKPLCEIEKLKHILGKARFIKLNNQWKGRCLHCWTTCMEDLLP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 23.4
• UniProt: P04019
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.