Protein Phosphatase 1 Regulatory Subunit 15A, Recombinant, Human, aa40-674, His-Tag

Product Overview

• Article Name: Protein Phosphatase 1 Regulatory Subunit 15A, Recombinant, Human, aa40-674, His-Tag
• Biozol Catalog Number: USB-586007
• Supplier Catalog Number: 586007
• Alternative Catalog Number: USB-586007-20,USB-586007-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on Ser-15. Source: Recombinant protein corresponding to aa40-674 from human Protein phosphatase 1 regulatory subunit 15A, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~33.7kD Amino Acid Sequence: SDEEEGEVKALGAAEKDGEAECPPCIPPPSAFLKAWVYWPGEDTEEEEDEEEDEDSDSGSDEEEGEAEASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSASTPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKETEEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSEKVTVHFLAVWAG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 33.7
• UniProt: O75807
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.