Purine Nucleoside Phosphorylase DeoD-type, Recombinant, E. coli, aa2-239, His-Tag

Product Overview

• Article Name: Purine Nucleoside Phosphorylase DeoD-type, Recombinant, E. coli, aa2-239, His-Tag
• Biozol Catalog Number: USB-586080
• Supplier Catalog Number: 586080
• Alternative Catalog Number: USB-586080-20, USB-586080-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Catalyzes the reversible phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Acts on 6-amino and 6-oxopurines including deoxyinosine, deoxyguanosine, deoxyadenosine, adenosine, guanosine, and inosine. Does not act on xanthosine. May also catalyze a phosphate-dependent transfer of the pentose moiety from one purine base to another. Source: Recombinant protein corresponding to aa2-239 from Escherichia coli Purine nucleoside phosphorylase DeoD-type, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~29.8kD Amino Acid Sequence: ATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 29.8
• UniProt: P0ABP8
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.