Replicase Large Subunit, Recombinant, Tobacco mosaic virus, aa829-1085, His-Tag

Product Overview

• Article Name: Replicase Large Subunit, Recombinant, Tobacco mosaic virus, aa829-1085, His-Tag
• Biozol Catalog Number: USB-586144
• Supplier Catalog Number: 586144
• Alternative Catalog Number: USB-586144-20, USB-586144-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

is an RNA-dependent RNA polymerase active in viral RNA replication., is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation. Source: Recombinant protein corresponding to aa829-1085 from Tobacco mosaic virus Replicase large subunit, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~32.6kD Amino Acid Sequence: VLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANSSGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYVYGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTSSVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYSDVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYT Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 32.6
• UniProt: O93058
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.