RNA Polymerase Sigma Factor for Flagellar Operon, Recombinant, E. coli, aa1-239

Product Overview

• Article Name: RNA Polymerase Sigma Factor for Flagellar Operon, Recombinant, E. coli, aa1-239
• Biozol Catalog Number: USB-586203
• Supplier Catalog Number: 586203
• Alternative Catalog Number: USB-586203-20,USB-586203-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor controls the expression of flagella-related genes. Source: Recombinant protein corresponding to aa1-239 from Escherichia coli RNA polymerase sigma factor for flagellar operon, expressed in E.coli. Molecular Weight: ~27.7kD Amino Acid Sequence: MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQAIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELVTDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTKLGKL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 27.7
• UniProt: P0AEM6
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.