Secreted Protein ORF2, Recombinant, Hepatitis E Virus Genotype 1, aa20-660, His-Tag

Product Overview

• Article Name: Secreted Protein ORF2, Recombinant, Hepatitis E Virus Genotype 1, aa20-660, His-Tag
• Biozol Catalog Number: USB-586257
• Supplier Catalog Number: 586257
• Alternative Catalog Number: USB-586257-20,USB-586257-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Plays a role in the inhibition of host antibody-mediated neutralization without blocking viral cell entry., Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5 end of the genomic RNA to mediate genome encapsidation. Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes. Source: Recombinant protein corresponding to aa20-660 from Hepatitis E virus genotype 1 Secreted protein ORF2, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~74.7kD Amino Acid Sequence: PPPGQPSGRRRGRRSGGSGGGFWGDRVDSQPFAIPYIHPTNPFAPNVTAAAGAGPRVRQPVRPLGSAWRDQAQRPAAASRRRPTTAGAAPLTAVAPAHDTPPVPDVDSRGAILRRQYNLSTSPLTSSVATGTNLVLYAAPLSPLLPLQDGTNTHIMATEASNYAQYRVARATIRYRPLVPNAVGGYAISISFWPQTTPTPTSVDMNSITSTDVRILVQPGIASELVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCIHGSPVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYSSTARHRLRRGADGTAELTTTAATRFMKDLYFTSTNGVGEIGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSRPVVSANGEPTVKLYTSVENAQQDKGIAIPNDIDLGESRVVIQDYDNQHEQDRPTPSPAPSRPFSVLRANDVLWLSLTAAEYDQSTYGSSTGPVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTIQQYSKIFFVLPLRGKLSFWEAGTTRPGYPYNYNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLGPHSALALLEDTLDYPARAHTFDDFCPECRPLGLQGCAFQSTVAELQRLKMKVGKTREL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 74.7
• UniProt: Q68985
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.